I-Ethylenediaminetetraacetic acid (EDTA) yintsimbi ye-chelating agent. Into ebizwa ngokuba yi-chelating ithetha ukuba inokudibanisa kunye nezinye ii-ion zetsimbi kwaye yenza ukuba ii-ion zetsimbi eziboshiweyo zingasebenzi.

Ukusebenzisa olu phawu lwe-EDTA, kukho iindidi ezimbini zezicelo kwiimvavanyo;

Olunye uhlobo lusetyenziso olubuyela umva, olunqanda ukusabela okuthile ngokuphazamisana nokubotshwa kweeyoni zetsimbi.

Thatha i-ion: Kwezinye ii-reactions, ukubotshwa kwee-ion zetsimbi kubalulekile. Sidibanisa i-EDTA kunye nezi ion zetsimbi ukunqanda le mpendulo.

Ngokomzekelo, i-coagulation yegazi idinga ukuthatha inxaxheba kwe-calcium ions. Songeza i-EDTA egazini ukuze i-chelate calcium ions kunye ne-EDTA, ngaloo ndlela ikhusela ukudibanisa kwegazi. Lo ngumgaqo we-EDTA anticoagulation;

Ngomnye umzekelo, ukulungiswa kwesisombululo se-decalcification kwi-pathological staining kusebenzisa umphumo we-chelation we-EDTA. Siyazi ukuba ukuba izicubu eziphezulu ze-calcium azinqunywanga, kuya kuba nzima ukusika kwiithishu ezipheleleyo xa usika, ngoko ke kufuneka sihlambulule ngaphambi kokuqhawula.

Kukho nohlobo lwesicelo sangaphambili esikhuthaza ukuphendulwa okuthile ngokuphazamisa ukubophelela kweeoni zetsimbi.

Umzekelo, yongeza i-EDTA kwisisombululo sokugaya i-trypsin. Ukuba i-EDTA ayongezwanga, i-trypsin iya kubophelela kwi-calcium kunye ne-magnesium kumphezulu weseli okanye kwindawo yenkcubeko, ngaloo ndlela inqanda umsebenzi we-trypsin.

Ngeli xesha, yongeza intwana ye-EDTA ukuvumela i-EDTA ukuba idityaniswe naloo calcium yasimahla, i-magnesium kunye nezinye ii-ion ze-divalent. Ngeli xesha, imeko entle yenziwa ukwenzeka kwe-trypsin.

Eyona nto imangalisayo nge-EDTA kukunyibilika kwayo. Inyibilika kancinane emanzini kwaye ibonakala imhlophe emanzini. Ngeli xesha, yongeza i-sodium hydroxide ukulungisa ixabiso layo le-pH. Njengoko i-pH iphakama kwaye iphezulu, isisombululo siba Siba sicace kwaye sicace. Xa i-pH isondele kwi-8, iyacaca ngokupheleleyo. Ayiloyiki iasidi kodwa ialkali.

I-EDTA inosetyenziso oluninzi olumangalisayo. Kuyabonakala ukuba izinto zendalo zinomlingo kakhulu, kodwa asizange siyiqaphele.

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