I-ethylenediaminetetraacetic acid (EDTA) yi-arhente ye-metal chelating. Oku kuthiwa yi-chelating kuthetha ukuba inokudibana nezinye ii-ion zesinyithi kwaye yenze ii-ion zesinyithi ezibotshelelweyo zingasebenzi.

Ukusebenzisa olu phawu lwe-EDTA, kukho iintlobo ezimbini zezicelo kwiimvavanyo;

Olunye uhlobo kukusetyenziswa okuchaseneyo, okuthintela iimpendulo ezithile ngokuphazamisana nokubopha kwee-ion zesinyithi.

Thatha i-ion: Kwezinye ii-reaction, ukubopha ii-ion zesinyithi kubalulekile. Sidibanisa i-EDTA nezi i-ion zesinyithi ukuthintela le reaction.

Umzekelo, ukujiya kwegazi kufuna ukuba ii-ion ze-calcium zithathe inxaxheba. Sifaka i-EDTA egazini ukuze sijiye ii-ion ze-calcium nge-EDTA, ngaloo ndlela sithintele ukujiya kwegazi. Lo ngumgaqo we-EDTA wokuthintela ukujiya kwegazi;

Umzekelo omnye, ukulungiswa kwesisombululo sokususa i-calcium kwi-pathological staining kukwasebenzisa i-chelation effect ye-EDTA. Siyazi ukuba ukuba izicubu ezine-calcium eninzi azisuswanga, kuya kuba nzima ukuzisika zibe zizicubu ezipheleleyo xa zisikwa, ngoko ke kufuneka sisuse i-calcium ngaphambi kokuba sinqumle.

Kukwakho nohlobo lokusetyenziswa phambili olukhuthaza iimpendulo ezithile ngokuphazamisana nokubopha kwee-ion zesinyithi.

Umzekelo, yongeza i-EDTA kwisisombululo sokugaya i-trypsin. Ukuba i-EDTA ayongezwanga, i-trypsin iya kubopha i-calcium kunye ne-magnesium kumphezulu weseli okanye kwindawo yokukhulisa, ngaloo ndlela ithintele umsebenzi we-trypsin.

Ngeli xesha, yongeza i-EDTA encinci ukuze i-EDTA idibane nezo calcium zasimahla, i-magnesium kunye nezinye ii-ion ezidivalent. Ngeli xesha, kwakheka imeko elungileyo yokwenzeka kwe-trypsin.

Eyona nto imangalisayo nge-EDTA kukunyibilika kwayo. Inyibilika kancinci emanzini kwaye ibonakala imhlophe emanzini. Ngeli xesha, yongeza i-sodium hydroxide ukuze ulungise ixabiso layo le-pH. Njengoko i-pH isanda, isisombululo siya sicaca ngakumbi nangakumbi. Xa i-pH isondele kwi-8, iba sobala ngokupheleleyo. Ayoyiki i-asidi kodwa i-alkali.

I-EDTA inemisebenzi emininzi emihle. Kuyabonakala ukuba izinto ezikwindalo zingumlingo kakhulu, kodwa asikayiboni loo nto.

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