1. I-Trihydroxymethyl aminomethane yolwalathiso lwe-industrial synthesis

Ngenxa yesakhiwo sayo esilula, i-athomu yekhabhoni ephakathi yi-tetrahedral structure, equlethe i-hydroxymethyl emithathu kunye neqela le-amino, elinokuthi liqhube iintlobo ezahlukeneyo zokutshintshwa kunye neempendulo ze-REDOX, luhlobo oluphezulu lwe-chemical synthesis raw materials. Enye yeendlela zokudibanisa i-buffer TAPS yenziwa ngokusebenzisa i-Tris kunye ne-1, i-3-propanolone kwi-alcohol. Ngokwesiqhelo, izinto ezikrwada ze-TRIS ezisetyenziselwa ukuhlanganiswa kwamashishini azifuni ukucoceka okuphezulu kwe-reagent. Ugxininiso kubuninzi obukhulu, ngoko kubiza kakhulu ukufumana umenzi wezinto eziluhlaza kunye nomthamo omkhulu.

2. I-Trimethylol amino methane yokukhangela ulwalathiso lwe-biochemical

I-Tris idla ngokusetyenziswa njengesithinteli sebhayoloji ukugcina i-pH yeempendulo ze-biochemical. Ngenxa yealkalinity ebuthathaka, iTris idla ngokusetyenziswa ngokudityaniswa neeasidi. Isetyenziswa ikakhulu kwi-nucleic acid, iprotein dissolution buffer, protein imprinting buffer, DNA imprinting experiment buffer, DNA agarose gel electrophoresis buffer, njalo njalo. iphezulu kakhulu, ngoko ke kufuneka ukhethe umenzi kunye neminyaka emininzi yophando kunye nophuhliso kunye namava emveliso, ukucoceka okuphezulu, ukungcola okuncinci ukuqinisekisa ukuchaneka kovavanyo lwe-biochemical. Ifuthe leqondo lobushushu le-Tris buffer, kulula ukufunxa ikharbon diokside emoyeni, uqwalaselo lwesithinteli kufuneka lugqunywe luvalwe ngokuqinileyo.

3. I-Trimethylol aminomethane yophando lwamachiza kunye nophuhliso

I-hydroxymethyl aminomethane enye i-alias ezintathu, ezizezi, i-trometamol, njengoko ingenayo isiseko se-sodium amino buffer, kwi-fluid yomzimba isabela nge-H2CO3, inciphisa i-H2CO3, ivelise i-HCO32 - kwakhona ngaxeshanye, inokufunxa ii-ion ze-hydrogen kunye ne-acidemia echanekileyo, umphumo wayo unamandla. , kwaye inokuthi nge-membrane yeseli, ehlala isetyenziswa kwi-acute metabolic kunye ne-accidemia yokuphefumula; Njengechiza le-alkaline, elisetyenziselwa ukulungiswa kwe-acidosis ngaphandle kokubangela ukwanda kokugcinwa kwekharbon diokside. Ngenxa yokuba isetyenziselwa amayeza, yonke imveliso ye-TRIS yomthombo wezinto ekrwada, indlela yenkqubo yokuvelisa, ukucoceka kwemveliso, umxholo we-ion wokungcola ziimfuno ezingqongqo, ukuthengwa kolu hlobo lweemveliso kufuneka kube ngqongqo kwaye kulumke, kufuneka kudlule uvavanyo olungqongqo.

4. I-Trimethylaminomethane isetyenziswa kwi-virus yokugcina ulwelo

Kwisisombululo sogcino lwentsholongwane, i-Tris inokugcina ixabiso le-pH lesampulu kuqala, lisebenza njengesithinteli sebhayoloji, kunye noluhlu lwe-pH buffer ye-Tris yile: 7.0 ukuya ku-9.0, ukukwazi ukugcina ukukhupha isampulu yokuqhekeka emva kokukhululwa ukuzinza kwe-nucleic acid, ukuphepha ukuthotywa kwe-nucleic acid, kunye nokuphucula ukugxila kunye nokucoceka kwe-nucleic acid, isiqinisekiso sokuchaneka kwemisebenzi elandelayo efana nohlalutyo lovavanyo lwe-nucleic acid, ngenxa yexesha elikhethekileyo lobhubhane, iimfuno zokufumanisa i-nucleic acid, i-Tris umthamo wokuthenga uya kuba mkhulu, ngoko ke cebisa ukuthenga iimveliso ezinjalo kufuneka kuqala kuthengwe isixa esincinci semveliso phambi kokuthenga isilingo esikhulu esiqinisekisiweyo ukuba akukho ngxaki, kwakhona kufuneka ibe nobugcisa emva kokuthengisa ukubonelela ngenkxaso yobuchwephesha.