1. I-Trihydroxymethyl aminomethane yolwalathiso lokwenziwa kwemizi-mveliso

Ngenxa yesakhiwo sayo esilula, i-athomu yekhabhoni ephakathi yi-tetrahedral structure, equlethe i-hydroxymethyl ezintathu kunye neqela le-amino, elinokwenza iintlobo ngeentlobo zokutshintshwa kunye neempendulo ze-REDOX, luhlobo lwezixhobo eziluhlaza zokwenziwa kweekhemikhali ezikumgangatho ophezulu. Enye yeendlela zokwenziwa kwe-buffer TAPS yenziwe kusetyenziswa iTris kunye ne-1, 3-propanolone kwi-alcohol. Ngokwesiqhelo, izixhobo eziluhlaza ze-TRIS ezisetyenziselwa ukwenziwa kwemizi-mveliso azifuni ubumsulwa obuphezulu be-reagent. Ugxininiso lukwinani elikhulu, ngoko ke kubiza kancinci ukufumana umenzi wezinto eziluhlaza onomthamo omkhulu.

2. I-Trimethylol amino methane yolwalathiso lokufumanisa izinto eziphilayo

I-Tris idla ngokusetyenziswa njenge-buffer yezinto eziphilayo ukugcina i-pH ye-biochemical reactions. Ngenxa yokuba ibuthathaka kwi-alkalinity, i-Tris idla ngokusetyenziswa kunye nee-asidi. Isetyenziswa kakhulu kwi-nucleic acid, i-protein dissolving buffer, i-protein imprinting buffer, i-DNA imprinting experiment buffer, i-DNA agarose gel electrophoresis buffer, njl. Kwinkqubo yokusabela ngenxa yokubandakanyeka kwee-enzymes, iiproteni kunye nee-antibodies ezahlukeneyo, ngoko ke iimfuno ze-buffer ziphezulu, ngoko ke ukuthenga kufuneka kukhethwe umenzi oneminyaka emininzi yophando nophuhliso kunye namava emveliso, ubunyulu obuphezulu, ukungcola okuncinci ukuqinisekisa ukuchaneka kweemvavanyo ze-biochemical. Isiphumo sobushushu be-Tris buffer, kulula ukufunxa i-carbon dioxide emoyeni, uqwalaselo lwe-buffer kufuneka lugqunywe ngokuqinileyo.

3. I-Trimethylol aminomethane yophando nophuhliso lwamayeza

I-hydroxymethyl aminomethane ezintathu yenye igama elingelolakhe, eliyi-trometamol, njengoko ingenaso isiseko se-amino buffer ye-sodium, kulwelo lomzimba isabela kwi-H2CO3, inciphisa i-H2CO3, ivelise i-HCO32 - kwakhona ngaxeshanye, inokufunxa ii-ion ze-hydrogen kwaye ilungise i-acidemia, isiphumo sayo sinamandla, kwaye sinokudlula kwi-membrane yeseli, esetyenziswa rhoqo kwi-acidemia ye-metabolic kunye ne-respiratory; Njengeyeza le-alkaline, elisetyenziselwa ukulungisa i-acidosis ngaphandle kokubangela ukwanda kokugcinwa kwe-carbon dioxide. Ngenxa yokuba isetyenziselwa amayeza, yonke imveliso ye-TRIS green material source, indlela yenkqubo yemveliso, ubunyulu bemveliso, umxholo we-ion wokungcola ziimfuno ezingqongqo, ukuthengwa kolu hlobo lweemveliso kufuneka kube ngqongqo kakhulu kwaye kulumke, kufuneka kudlule uvavanyo olungqongqo.

4. I-Trimethylaminomethane isetyenziswa kulwelo lokugcina iintsholongwane

Kwisisombululo sokulondolozwa kwentsholongwane, iTris inokugcina ixabiso le-pH yesampuli kuqala, isebenza njenge-buffer yebhayoloji, kwaye uluhlu lwe-pH buffer yeTris lulandelayo: 7.0 ukuya kwi-9.0, ukukwazi ukugcina ukuqhekeka kwesampuli emva kokukhululwa kozinzo lwe-nucleic acid, ukuphepha ukonakala kwe-nucleic acid, kunye nokuphucula uxinaniso kunye nobunyulu be-nucleic acid, ukuqinisekisa ukuchaneka kwemisebenzi elandelayo efana nohlalutyo lovavanyo lwe-nucleic acid, ngenxa yexesha elikhethekileyo lobhubhane, iimfuno zokufunyanwa kwe-nucleic acid, ubungakanani bokuthenga beTris buya kuba bukhulu, ngoko ke cebisa ukuba uthenge iimveliso ezinjalo kufuneka kuqala uthenge inani elincinci lemveliso ngaphambi kokuthenga uvavanyo olukhulu oluqinisekisiweyo ukuba akukho ngxaki, kufuneka kwakhona ube nobuchwephesha emva kokuthengisa ukubonelela ngenkxaso yobuchwephesha yobungcali.